Alternative splicing analysis benchmark with DICAST

Abstract

Alternative splicing is a major contributor to transcriptome and proteome diversity in health and disease. A plethora of tools have been developed for studying alternative splicing in RNA-seq data. Previous benchmarks focused on isoform quantification and mapping. They neglected event detection tools, which arguably provide the most detailed insights into the alternative splicing process. DICAST offers a modular and extensible framework for the analysis of alternative splicing integrating 11 splice-aware mapping and eight event detection tools. We benchmark all tools extensively on simulated as well as whole blood RNA-seq data. STAR and HISAT2 demonstrated the best balance between performance and run time. The performance of event detection tools varies widely with no tool outperforming all others. DICAST allows researchers to employ a consensus approach to consider the most successful tools jointly for robust event detection. Furthermore, we propose the first reporting standard to unify existing formats and to guide future tool development.

Publication
bioRxiv
Amit Fenn
Amit Fenn
Postdoctoral Researcher at Helmholtz Munich
Olga Tsoy
Olga Tsoy
Group Leader
Tim Faro
Tim Faro
Student research assistant
Fanny Rößler
Fanny Rößler
Student research assistant
Zakaria Louadi
Zakaria Louadi
Researcher at Illumina Artificial Intelligence Laboratory
Chit Tong Lio
Chit Tong Lio
Postdoctoral researcher